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Flora and Vegetation of Asian Russia

2019 year, number 4

1.
ANATOMO-MORPHOLOGICAL FEATURES OF LEAF OF HEDYSARUM ALPINUM (FABACEAE) IN BURYATIA

N.A. Karnaukhova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
karnaukhova-nina@rambler.ru
Keywords: Hedysarum alpinum, Fabaceae, анатомия листа, размеры и число клеток, устьиц, идиобластов, ксероморфные и мезоморфные признаки, Бурятия, Hedysarum alpinum, Fabaceae, anatomy leaf, size and number of cells, stomata, idioblasts, xeromorphic and mesomorphic features, Buryatia

Abstract >>
The adaptation of Hedysarum alpinum to different growth conditions in Buryatia is traced by anatomical features of the leaf blade structure. Structural and quantitative changes in the anatomical structure of the leaf are analyzed, and their score is given according to the value of each trait as xeromorphic or mesomorphic.



2.
VARIABILITY MALE GAMETOPHYTE LONICERA CAERULEA (CAPRIFOLIACEAE) IN NATURAL POPULATIONS OF GORNY ALTAI

A.I. Kluchevskaya, I.G. Boyarskikh
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
irina_2302@mail.ru
Keywords: Lonicera caerulea, пыльца, фертильность, изменчивость, популяции, Горный Алтай, зоны разломов, Lonicera caerulea, pollen, fertility, variability, populations, Altai Mountains, fault zone

Abstract >>
Variability of pollen fertility and morphometric parameters in natural populations of Lonicera caerulea L. Gorny Altai was studied. Pollen of Lonicera caerulea was characterized by high fertility in various ecological and geographical conditions of the mountain system. Reducing the fertility of pollen grains and a signifi cant change in their morphometry in individual populations of L. caerulea subsp. altaica , located in areas with anomalous geophysical characteristics under the influence of a complex of undifferentiated geological and geophysical factors associated with seismotectonic activity has been established.



3.
THE SYNTAXONOMY OF THE XERIC MEADOWS OF BARNAUL PINE FOREST STRIP (ALTAI TERRITORY)

M.P. Tishchenko, A.Yu. Korolyuk
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
tishenko-1957@mail.ru
Keywords: остепненные луга, синтаксономия, ленточные боры, Алтайский край, xeric meadows, syntaxonomy, strip pine forests, Altai territory

Abstract >>
On the basis of 132 relevés the syntaxonomical diversity of xeric meadows from Barnaul pine forest strip was described. It is represented by 2 associations, 3 subassociations, 1 variant and 1 community from order Brachypo dietalia pinnati of the class Festuco-Brometea . Two sytaxa were firstly described. Spatial distribution of meadow communities in forest strip structure was shown.



4.
PLANT COMMUNITIES FORMATION IN INITIAL STAGES OF SPONTANEOUS VEGETATION ON ASH DUMPS WITH A FERTILE LEVEL APPLIED (TPS-5, Novosibirsk)

N.V. Sheremet, T.G. Lamanova, V.M. Doronkin
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
nsheremet@yandex.ru
Keywords: золоотвалы, нанесенный плодородный слой почвы, растительные сообщества, естест венное зарастание, лесостепь, ТЭЦ, ash dumps, fertile layer, plant communities, natural revegetation, forest-steppe, Thermal Power Station

Abstract >>
The article presents the results of an original research on the specific of spontaneous vegetation on ash dumps in the forest-steppe zone in the south of West Siberia (Thermal Power Station 5, Novosibirsk, Russia) during the initial 9 years. The grass vegetation prevails: we detected 85 grass species, 8 tree species and 6 moss species. Th e dominating species according to the above-ground phytomass are grasses Phleum pratense , Dactylis glomerata and Agrostis gigantea ; amongst the legumes, Trifolium hybridum and T. pratense ; amongst the forbs, Artemisia dracun culus , A. vulgaris and Cirsium setosum. The vertical structure of the plant communities has the extended type. Th e air-dry phytomass was estimated as 2080 kg/ha for the bent grass-clover community, 3060 kg/ha for the forbsclover-grass community, and 5090 kg/ha for forbs-legumes-grass community with willow and sea buckthorn bushes. The above-ground phytomass exceeds the below-ground one in two communities; the values are equal for the forbs-clover-grass community.



5.
INTRODUCTION OF REPRESENTATIVES OF THE GENUS ACER IN THE NOVOSIBIRSK

A.P. Belanova, L.N. Chindyaeva, E.M. Lyakh
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
boronina.a@inbox.ru
Keywords: виды рода Acer, фенологические исследования, дендрологические коллекции, городская среда, Acer species, phenological study, dendrological collections, urban environment

Abstract >>
The article deals with the characteristic of Acer species introduced in Novosibirsk and the recommendations on rational use of maples in urban environment. Study of 14 species of the genus Acer shows that A . ginnala and A. tataricum should be recommended for wider use in the city and A . spicatum , A. tegmentosum , A. mono , A. mandshuricum and A. barbinerve for limited use in gardens, parks, andresidential areas. At present A. campestre , A. pseudosieboldianum , A. semenovii show little promise due to low winter hardiness. It is essential to restrict application of the invasive species A . negundo , it should be only used near interregion motorways.



6.
INFLUENCE OF ARTIFICIAL DEFOLIATION ON TRYPSIN INHIBITOR ACTIVITY IN HEDYSARUM THEINUM (FABACEAE)

E.V. Zhmud, I.N. Kuban, A.A. Achimova, M.B. Yamtyrov, O.V. Dorogina
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
elenazhmu@ngs.ru
Keywords: трипсинингибирующая активность, морфометрические признаки, Hedysarum theinum, фенологические фазы сезонного развития, природные условия, культура, trypsin inhibitor activity, morphometric features, Hedysarum theinum, phases of seasonal development, natural conditions, cultivation

Abstract >>
Trypsin inhibitor activity (TIA) is one of the of immunity factors, that provide ensures the presence of general nonspecific systemic resistance in angiosperm plants. In the experiment with artificial defoliation, the variability of TIA in leaves of the Siberian endemic species Hedysarum theinum Krasnob. ( Fabaceae ) has been studied. Th is is due to its use as a medicinal and fodder. It is shown that TIA in H. theinum leaves in defoliation can be one of the criteria for the adaptive capabilities of the species.



7.
IN VITRO MORPHOGENIC POTENTIAL OF FRITILLARIA MELEAGRIS F. ALBA (LILIACEAE) FLORAL ORGANS

D.S. Muraseva, T.V. Zheleznichenko, T.I. Novikova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
dsmuraseva@csbg.nsc.ru
Keywords: Fritillaria meleagris f. alba, культура ткани, морфогенез, регуляторы роста растений, первичный эксплант, регенерация, листочек околоцветника, тычинка, пестик, Fritillaria meleagris f. alba, tissue culture, morphogenesis, plant growth regulators, primary explants, regeneration, tepals, stamen, pistil

Abstract >>
For the first time, the morphogenic potential of three types of Fritillaria meleagris floral explants - tepals, stamens, pistils - was studied to identify the most competent organs for in vitro culture initiation. It has been established, that plant growth regulators and their concentrations were the most significant factors inducing the regeneration process in the explant tissues. B5 nutrient medium supplemented with BA 0.4 μM, NAA 3.2 μM and IAA 2.3 μM was the most efficient for morphogenesis induction. A tenfold increase of BA content in this media inhibited shoot formation. Using a stereomicroscopy, it was observed that the morphogenesis processes localizes in the proximal parts of the tepals and filaments. Whereas in pistil culture regeneration process occurred across the ovary surface. A comparative assessment of the morphogenic potential revealed that tepals possessed the highest regenerative capacity among the studied types of primary explants.



8.
CULTIVATION OF HAIRY ROOTS OF VALUABLE MEDICINAL PLANT ASTRAGALUS PENDULIFLORUS (FABACEAE) IN GAS-VORTEX BIOREACTOR "VORTEXLAB-10"

E.V. Ambros, T.A. Kukushkina, T.I. Novikova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
ambros_ev@mail.ru
Keywords: Fabaceae, Astragalus penduliflorus, hairy roots, bioreactor "VortexLab-10", Gamborg-Eveleg’s medium, tannins, triterpenoid saponins, carotenoids

Abstract >>
The cultivation of Astragalus pendulifl orus Lam. hairy roots in the gas-vortex bioreactor “VortexLab-10” in liquid Gamborg-Eveleg medium was tested for the first time. It was shown that under the conditions of the bioreactor, cultures retained the ability to synthesize biologically active compounds (BAC) specific for this species with maintaining the growth activity ( I = 13.2). In addition, the using of gas-vortex bioreactor allowed to increase the content of individual BAC in hairy roots in comparison with shaker culture. The content of tannins increased 2.9 times, carotenoids - 5.9 times.



9.
DIGITAL HERBARIUM OF THE CSBG SB RAS IN GLOBAL BIODIVERSITY INFORMATION FACILITIES

N.K. Kovtonyuk, I.V. Han, E.A. Gatilova, Yu.A. Pshenichkina
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
nkovtonyuk@csbg.nsc.ru
Keywords: биоресурсные коллекции, глобальная информационная система (GBIF) по биоразнообра зию (IPT), датасет, редкие растения, флора России, цифровой гербарий, Allium, Geranium, Medicago, Rhododendron, Primulaceae, bioresource collections, Global Biodiversity Information Facility (GBIF), IPT, dataset, rare plants, Flora of Russia, Digital Herbarium, Allium, Geranium, Medicago, Rhododendron, Primulaceae

Abstract >>
The Digital Herbarium of the Central Siberian Botanical Garden of SB RAS began to be created on the taxonomic basis in 2017. Completely digitized herbarium specimens of genera Allium , Geranium , Medicago , Rhododendron , family Primulaceae , rare plants of Siberian flora, as well as type specimens of vascular plants stored in NS and NSK collections at the CSBG SB RAS. The digitization results are published in the open access as 6 datasets on the Global Biodiversity Information Facility (GBIF) portal using the Integrated Publishing Toolkit (IPT) installed on the CSBG server. Digitization is performed on two ObjectScan 1600 scanners (Microtek) using soft ware ScanWizard-Botany and MiVapp-Botany according to the international standards, at 600 dpi, with a barcode, 24-color scale and spatial scale bar. The Digital Herbarium is created as a network resource on biodiversity, the materials of which can be used in GBIF, for taxonomic treatments in the preparation of “Flora of Russia”, in various types of scientific research and educational purposes.