IN VITRO MORPHOGENIC POTENTIAL OF FRITILLARIA MELEAGRIS F. ALBA (LILIACEAE) FLORAL ORGANS
D.S. Muraseva, T.V. Zheleznichenko, T.I. Novikova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
dsmuraseva@csbg.nsc.ru
Keywords: Fritillaria meleagris f. alba, культура ткани, морфогенез, регуляторы роста растений, первичный эксплант, регенерация, листочек околоцветника, тычинка, пестик, Fritillaria meleagris f. alba, tissue culture, morphogenesis, plant growth regulators, primary explants, regeneration, tepals, stamen, pistil
Abstract
For the first time, the morphogenic potential of three types of Fritillaria meleagris floral explants - tepals, stamens, pistils - was studied to identify the most competent organs for in vitro culture initiation. It has been established, that plant growth regulators and their concentrations were the most significant factors inducing the regeneration process in the explant tissues. B5 nutrient medium supplemented with BA 0.4 μM, NAA 3.2 μM and IAA 2.3 μM was the most efficient for morphogenesis induction. A tenfold increase of BA content in this media inhibited shoot formation. Using a stereomicroscopy, it was observed that the morphogenesis processes localizes in the proximal parts of the tepals and filaments. Whereas in pistil culture regeneration process occurred across the ovary surface. A comparative assessment of the morphogenic potential revealed that tepals possessed the highest regenerative capacity among the studied types of primary explants.
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