CULTIVATION OF HAIRY ROOTS OF VALUABLE MEDICINAL PLANT ASTRAGALUS PENDULIFLORUS (FABACEAE) IN GAS-VORTEX BIOREACTOR "VORTEXLAB-10"
E.V. Ambros, T.A. Kukushkina, T.I. Novikova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
ambros_ev@mail.ru
Keywords: Fabaceae, Astragalus penduliflorus, hairy roots, bioreactor "VortexLab-10", Gamborg-Eveleg’s medium, tannins, triterpenoid saponins, carotenoids
Abstract
The cultivation of Astragalus pendulifl orus Lam. hairy roots in the gas-vortex bioreactor “VortexLab-10” in liquid Gamborg-Eveleg medium was tested for the first time. It was shown that under the conditions of the bioreactor, cultures retained the ability to synthesize biologically active compounds (BAC) specific for this species with maintaining the growth activity ( I = 13.2). In addition, the using of gas-vortex bioreactor allowed to increase the content of individual BAC in hairy roots in comparison with shaker culture. The content of tannins increased 2.9 times, carotenoids - 5.9 times.
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