PILOT STUDY OF THE ASSOCIATION OF TRPA1 AND TRPV1 GENE POLYMORPHISMS WITH MYOCARDIAL INFARCTION
P.S. Orlov1,2,3, V.N. Maksimov1,2,3, S.V. Mikhaylova2, D.E. Ivanoshchuk1,2,3, S.K. Malyutina1, M.I. Voevoda1,2,3,4
1Research Institute of Therapy and Preventive Medicine - Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS, 630089, Novosibirsk, Boris Bogatkov str., 10
2Federal Research Center Institute of Cytology and Genetics of SB RAS, 630090, Novosibirsk, Akademik Lavrentiev av., 10
3Novosibirsk State University, 630090, Novosibirsk, Pirogov str., 2
4Federal Research Center for Fundamental and Translational Medicine, 630117, Novosibirsk, Timakov str., 2
Keywords: инфаркт миокарда, TRPA1, TRPV1, rs222747, rs13268757, myocardial infarction, TRPA1, TRPV1, rs222747, rs13268757
Abstract
The aim of the study was to investigate the association of SNPs in the TRPA1 (rs13268757) and TRPV1 (rs222747) genes with myocardial infarction. Material and methods. Myocardial infarction (MI) (200 individuals) and control (420 individuals) groups were formed as a part of international HAPIEE project. Genotyping the groups for the studied rs13268757 and rs222747 polymorphisms was performed by a real-time PCR on a StepOnePlus device (Applied Biosystems, USA) using TaqMan probes (Applied Biosystems, USA) according to a standard protocol. Intergroup comparison of the allele/genotype frequencies for each of the studied polymorphisms was calculated using Fisher’s exact test and the SPSS 11.0 program. The correspondence of genotype frequencies to Hardy-Weinberg equilibrium was tested using the χ2 method. The relative risk of MI for a particular allele or genotype was calculated as an odds ratio (OR) using Fisher’s exact two-sided test and Pearson chi-square test. Differences were considered statistically significant with a significance level p <0.05. Results. The frequency of the homozygous AA genotype for the TRPA1 gene rs13268757 polymorphism differed significantly in patients with MI as compared with the control, AA vs GG + AG OR = 2.621 (95 % CI 1.112-6.175; p = 0.034). No statistically significant results were shown for the TRPV1 rs222747. Conclusion. The association of the TRPV1 gene rs222747 and TRPA1 gene rs13268757 polymorphisms with MI was for the first time checked in Russia. The association with MI was shown for the TRPA1 gene rs13268757 polymorphism.
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