Autochthonous Bacteriophages in the Structure of the “Microbial Loop” in Different Biotopes of Lake Baikal
V. V. DRUCKER1, O. I. BELYKH1, A. S. GORSHKOVA1, A. A. BONDAR2, N. N. SYKILINDA3
1Limnological Institute, SB RAS, Irkutsk, Ulan-Batorskaya str., 3
2Genomic Core Facility, Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Acedemican Lavrentyev ave., 8
3Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Miklukho-Maklaya str., 16/10
Keywords: бактериофаги, Байкал, морфотипы, численность, планктон, нейстон, биопленки, bacteriophages, Lake Baikal, morphotypes, abundance, plankton, neuston, biofilms
Abstract
The paper presents results of study of autochthonous bacteriophages - previously unknown element in the structure of the “microbial loop” in the ecosystem of deep oligotrophic Lake Baikal. Transmission electron microscopy method was applied to study morphological diversity of phages in the water column, surface microlayer and benthic biofilms at the water-rock (marble, granite, mica, quartz, amphibolite, gabbro, urtite) interface. Data on the size structure of the autochthonous bacteriophages, their quantity, seasonal dynamics and vertical distribution from the surface to the maximum depth of 1200 m in comparison with bacteria are given. A few morphotypes of Baikal autochthonous bacteriophages affecting Pseudomonas aeruginosa are isolated and characterized by means of molecular and genetic methods. Full genomes of a gigantic phage PaBG of the family Myoviridae and MD8 of the family Siphoviridae are determined.
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