VARIABILITY OF NEEDLE ANTIMICROBIC ACTIVITY IN CLONE PLANTATION OF SCOTS PINE Pinus sylvestris L
V. V. Tarakanov1,2, L. N. Chindyaeva3, N. V. Tsybulya3, I. V. Tikhonova4
1V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Zukovsky str., 100/1, Novosibirsk, 630082 Russian Federation
2Novosibirsk State Agrarian University, Dobrolyubov str., 160, Novosibirsk, 630039 Russian Federation
3Central Siberian Botanical Garden, Russian Academy of Sciences, Siberian Branch, Zolotodolinskaya str., 101, Novosibirsk, 630090 Russian Federation
4Solitary Unit V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
Keywords: межклоновая изменчивость сосны обыкновенной, антимикробная активность, микробные тест-объекты, метод опарения, between clonal variability of Scots pine, antimicrobial activity, microbe test objects, the pair method
Abstract
Streak inoculation of microbial test-cultures was treated with volatile emissions of needles of 23-years clones of Pinus sylvestris L. The study was conducted in July 2011 on three test-objects - gram-positive bacteria Staphyllococcusepidermidis, gram-negative bacteria Esсherichia coli and yeast-like fungi Candida albicans. Antimicrobial effect of pine volatile emissions was assessed in comparison with the control on 0 (absence of the effect) - 4 (strong effect) scale of antimicrobial activity. The mean marks of antimicrobial activity of clones, calculated in relation to separate test-systems, were within the limits of 0.00-3.00. The weak differentiation of pine clones to S. epidermidis is revealed. Frequency of pair comparisons in which statistically significant differences between clones was found to be less than 5 % for S. epidermidis and about 1.5 % for all the test-microbes. Alongside with it significant effects of the time of testing (calendar dates), a microbial species («pathogens») and interactions «date of testing-pathogens» and «date of testing-clones» were revealed: in ANOVA their effects amounted to nearly 23, 33 and 15 % of the total dispersion accordingly. The most probable reasons for it are dynamics of structure of essential oils and «genotype-environment» interaction that should become object of future research. In this connection, an important question is what is the season that is optimum for reliable revealing of the pine genotypes with maximal antimicrobial activity? Negative between-clones (between-genotypes) correlations between antimicrobial activity to S. epidermidis and to C. albicans are revealed. It is probably caused by inversely proportional dependence between concentrations of the components in essential oils, which are responsible for inhibition of various pathogenic organisms. The sample of investigated clones was divided into two sub-samples that sufficiently differed by the levels of both antimicrobial activity to S. epidermidis and individual heterozygosity of allozyme’s loci. Such differentiation of genotypes is noted for the first time, and can be in focus of further studies.
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