Drought Impact on Wood Formation and Antioxidant Protection of Scots Pine Cambial Zone
N. E. Sudachkova, L. I. Romanova, I. L. Milyutina
V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
Keywords: сосна обыкновенная, засуха, ксилогенез, камбий, флоэма, фотосинтетические пигменты, углеводы, окислительный стресс, Scots pine, drought, xylogenesis, cambium, phloem, photosynthetic pigments, carbohydrates, oxidative stress
Abstract
The effect of drought on the 8-9-year-old seedlings of Scots pine (
Pinus sylvestris L.) was simulated in the field by isolating trees from precipitation. The biochemical changes typical of water stress wеre compared with the structural changes of the annual rings of wood. The samples of the current and last year needles, cambium and adjoining layers of xylem and phloem of stems and roots were analyzed. In the needles, the content of chlorophyll a and b and carotenoids were determined. The contents of hydrogen peroxide and malondialdehyde (MDA), the activity of enzymes, realizing antioxidant protection: superoxide dismutase (SOD), peroxidase, glutathione reductase and content of sugars and starch were determined in tissues of xylem, phloem and cambium. It was shown that drought reduces the weight of the needles, the chlorophyll content and the width of the annual wood rings. In moderate drought in the cambial zone oxidative stress was developed and also protection system against free radicals was activated, which resulted in a high SOD activity and the accumulation of hydrogen peroxide due to the activity of peroxidase reduction. As a result, the division processes in cambial zone and cells extension growth were inhibited and the width of annual wood rings decreased due to reduction in the number and size of tracheids. It was found that decrease in growth was not due to deficit of carbohydrates for the process xylogenesis. Water deficit increases the concentration of low molecular weight carbohydrates in the tissues, which, due to the inhibition of division and extension cambial derivatives are only partially used for thickening tracheid cell walls. As a result, abnormal tracheides with reduced size of cells and lumens and thickened cell walls were formed. Abundance of soluble carbohydrates was deposited as a reserve pool in the root phloem in the form of starch. The stock function of root phloem was increased under water deficit conditions.
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