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Contemporary Problems of Ecology

2004 year, number 2

Molecular genetic approach to the investigation of processes of plasmid spreading over natural microbe populations



Molecular-ecological aspects of investigation and monitoring of transgenic microorganisms under their purposeful or accidental introduction into environment are discussed. Special attention is paid to the problems connected with the attachment of recombinant plasmids in the link of aboriginal microorganisms. The problem concerning the possibility to attach natural plasmids in the cells of transgenic microorganisms is also discussed. A methodological approach, which includes microbiological, genetic and molecular aspects, is proposed for experimental estimations of the potential risk of the spreading of recombinant plasmids and transgenic microorganisms in natural ecosystems.