COMPARATIVE ANALYSIS OF MITOCHONDRIAL DNA copy numberS IN MYOCARDIAL TISSUE IN sudden Cardiac AND NON-Cardiac DEATH
V.N. Maksimov1,2, A.A. Gurazheva3, P.S. Orlov3, S.K. Malyutina1,2, A.A. Ivanova3, S.V. Maksimova4, I.A. Rodina5, O.V. Khamovich5, V.P. Novosyolov4,5
1Research Institute of Internal and Preventive Medicine - Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS, 630089, Novosibirsk, Boris Bogatkov str., 175/1, 630091, Novosibirsk, Krasny av., 52
2Novosibirsk State Medical University of Minzdrav of Russia
3Research Institute of Internal and Preventive Medicine - Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS, 630089, Novosibirsk, Boris Bogatkov str., 175/1
4Novosibirsk State Medical University of Minzdrav of Russia, 630091, Novosibirsk, Krasny av., 52
5Novosibirsk Regional Clinical Bureau of Forensic Medicine, 630087, Novosibirsk, Nemirovich-Danchenko str., 134
Keywords: количество копий митохондриальной ДНК (мтДНК), фактор риска, атеросклероз, ИБС, инфаркт миокарда, внезапная сердечная смерть (ВСС), популяция, mitochondrial DNA (mtDNA) copy number, risk factor, atherosclerosis, IHD, myocardial infarction, sudden cardiac death (SCD), population
Abstract
The aim of the work is to compare the number of copies of mtDNA in myocardial tissue in persons who died of sudden cardiac and non-cardiac death. Material and methods. Group of sudden cardiac death (SCD, 150 samples): the autopsy material was collected from those who suddenly died outside the medical and preventive treatment facilities of persons who underwent a forensic medical examination according to a standard protocol. As a control group (n = 150), a sample of individuals (selected by gender and age) was used, which, according to the conclusion of the forensic medical examination, died suddenly from other causes (SD). The study of the number of copies of mtDNA was performed in DNA samples isolated from myocardial tissue by the method of phenol-chloroform extraction, using real-time quantitative PCR (qPCR). Results. In both studied groups, there are no significant correlations of the number of copies of mtDNA with age in both men and women. In the general regression analysis, with the introduction of age into the model, the difference between the SCD and SD groups was obtained in the number of copies of mtDNA (p = 0.01). When divided by sex in the group of women, there are no differences between SCD and SD in the number of copies of mtDNA (p = 0.089). In men, differences in the number of copies of mtDNA persist between SCD and SD (p = 0.023). High variability in the number of copies of mtDNA in the myocardium was noted even within the same group in individuals of the same sex and of comparable age compared to the variability in the number of copies of mtDNA in peripheral blood leukocytes (according to the literature). This is probably due to the heterogeneity of the groups on the etiology and pathogenesis of sudden death. In men, the decrease in the number of copies of mtDNA in the group with SCD, compared with those who died suddenly from other causes, is more pronounced at the age of 50 years and older. Conclusion. The number of copies of mtDNA in myocardial tissue in men who have died of SCD is lower, compared with men who died suddenly from other causes (especially over the age of 50 years).
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