THE GENERATION OF INTERSPECIFIC HYBRIDS IN (Populus alba x P. bolleana) x P. x сanescens BY in vitro CULTURE
A. A. Erst, S. V. Shishkin, M. S. Voronkova
Central Siberian Botanical Garden, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russian Federation
Keywords: тополя белый, Болле, сереющий, сибирский серебристый, гибридное потомство, морфогенез in vitro, БАП, poplars white, Bollea, gray, Siberian silver poplar, hybrid progeny, morphogenesis in vitro, BAP
Abstract
Eight variants of the Siberian silver poplar hybrid N. 12 (Populus alba × P. bolleana) × P. × canescens obtained by the method of hybridization on the harvested branches were studied. 100 % germination of in vitro hybrid seeds was noted. According to the type of morphogenic response, multiplication factor, the parameters of growth and development in vitro and ex vitro, there was a high variability in the characteristics of the variants obtained. Variants N. 1, 6 and 8 were characterized by the development of a conglomerate of adventive buds at the base of shoots in response to the presence of 0.5-2.5 µM 6-benzylaminopurine (BAP) in the nutrient medium, for other variants the growth of the base of the shoot and the lower leaves was followed by the formation of adventive buds. The maximum multiplication factor (20.1 pss/exp) was obtained for variant N. 8 on a medium with 2.5 µM BAP. The use of a BAP concentration of more than 2.5 μM had a negative effect, since it only led to a significant expansion of the shoot bases, lower leaves and a decrease in the multiplication factor. 100 % rhizogenesis was observed on a hormone-free medium 1/2 MS. For variant N. 8, an increased accumulation of anthocyanins by the root system was noted. When grown under ex vitro conditions, differences in the shape of the leaf plate of the hybrids were observed, as option variant N. 8 was characterized by the presence of leaves without lobes. It is shown that the combination of classical hybridization methods and in vitro methods allows the maximum use of the genetic potential of new poplar hybrids in breeding.
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