Pilot Testing and Assessment of Microbial Contamination of Hydraulic Fluid Based on Hydrotol-ITCh HFAE Concentrate for Hydraulic Systems of Powered Mine Supports
V. A. VALTSIFER1, M. V. KUZNETSOVA2, A. V. ZAITSEV3, I. V. VALTSIFER1, E. V. SAENKO1, L. YU. NESTEROVA2, L. YU. LEVIN3
1Institute of Technical Chemistry Ural Branch Russian Academy of Sciences, Perm, Russia
2Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences, Perm, Russia
3Mining Institute Ural Branch Russian Academy of Sciences, Perm, Russia
Keywords: hydraulic concentrate, hydraulic fluid, operating parameters, microbial contamination, 16S rRNA sequencing
Pages: 97-107
Abstract
Pilot-scale tests results and laboratory studies of fire-resistant hydraulic fluid based on the developed hydraulic concentrate Hydrotol-ITCh HFAE are presented. Stable operation and operating parameters preservation of the developed hydraulic fluid physicochemical properties under the real conditions on a contaminated hydraulic system of a mining industry enterprise are shown. It was found that during six months of operation, the range of pH values, electrical conductivity, concentration and bacteriological purity did not change significantly, while the concentrate refilling, as well as the use of special disinfectants and cleansers, was not required. For the first time, the microbial composition of hydraulic fluid exposed to operation in the powered supports of the enterprise was studied using the method of metagenomic sequencing. Molecular genetic methods revealed a wide range of bacterial associates with various types of metabolism, including anaerobic sulphate-reducing bacteria involved in the biological corrosion of metals.
DOI: 10.15372/CSD2024536
EDN: WKVMDD
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