Impact of Genetic Modification of the Antioxidant Defense of Tobacco Plants on the Streptomycetes Complex in the Rhizosphere
I. G. SHIROKIKH1,2, Ya. I. NAZAROVA1, A. A. SHIROKIKH1, S. Yu. OGORODNIKOVA2, E. V. TOVSTIK1, E. N. BARANOVA3
1N. V. Rudnitski Zonаl Research Institute for Agriculture of the North-East, 610007, Kirov, Leninа str., 166а
2Vyatka State University of Humanities, 610002, Kirov, Krasnoarmeyskaya str., 26
3All-Russian Research Institute of Agricultural Biotechnology, 127550, Moscow, Timiryazevskaya str., 42
Keywords: soil, streptomycetes, structure of the complex, rhizosphere, tobacco, transformant plant, Fe superoxide dismutase (Fe-SOD1), oxidative stress
Abstract
Tobacco plants (Nicotianа tabacum L.) modified with the gene of Fe superoxide dismutase (Fe-SOD1) from Arabidopsis thaliana L. were studied. The gene of Fe-SOD1 conferred resistance to the plants against the damaging effects of oxidative stress. The number and structure of complexes of actinomycetes of the genus Streptomyces in the rhizosphere and rhizoplane of the original cultivar “Samsun” and independent transgenic lines Ttrf3 and Ttrf13 were estimated. The plants were grown under optimal conditions (control) and on acid sod-podzolic soil with aluminum (stress). The impact of incorporation of the heterologous sequence in the genome of tobacco plants on the abundance, species diversity and functional activity of streptomycetes in the rhizosphere of the plants was detected.
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