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Flora and Vegetation of Asian Russia

2016 year, number 4

EFFICIENCY OF APPLICATION OF EMBRYO CULTURE METHOD FOR BREAKING DORMANCY IN NITRARIA SIBIRICA (NITRARIACEAE) SEEDS

T.V. Zheleznichenko, T.I. Novikova, E.V. Banaev
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
zhelez05@mail.ru
Keywords: Nitraria sibirica, культура in vitro, прорастание семян, околоплодник, скарификация, стратификация, эмбриокультура, покой семян, Nitraria sibirica, in vitro culture, germination of seeds, pericarp, scarifi cation, stratifi cation, embryo culture, seed dormancy

Abstract

Th e effects of different pretreatments, including stratification, chemical scarifi cation (with H2SO4) , application of gibberelic acid (GA3), combination of chemical scarification with GA3 application or embryo culture method, on in vitro seed germination were studied . The general germinating ability, energy and dynamics of germination were analyzed. The best conventional method of treatment was combination of chemical scarification and application of GA3, while seedlings emerged after a week of incubation and the overall germination amounted to 27.54 %. Th e embryo culture method using after short of chemical scarification lead to first seedlings formation on the third day of cultivation and a multitude of seedlings was noted on the 4th or 5th days. The overall germination amounted to 39.2 % in embryo culture, which demonstrated the high efficiency of biotechnological approaches.