EVALUATION OF ISSR ANALYSIS FOR STUDYING OF THE GENUS TROLLIUS (RANUNCULACEAE) DIVERSITY
L.V. Buglova, N.S. Nuzhdina
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya srt., 101
astro11@rambler.ru
Keywords: Trollius, ISSR, кластерный анализ, метод главных компонент, виды, интрогрессия, морфологические признаки, Trollius, ISSR, cluster analysis, principal component analysis, species, introgression, morphological fea tures
Abstract
The ISSR fingerprinting of the twelve Trollius species ( Ranunculaceae family) was used in order to establish its inter-species relationships. Based on 116 ISSR markers obtained by PCR amplification with four ISSR primers three divergent groups of species were revealed: european, siberian and far-eastern. According to the ISSR data the species T. altaicus , T. asiaticus , T. kytmanovii , T. sajanensis , have close genetic relationships, therefore introgressive hybridization between these species is permitted. The greatest genetic distance ( D = 0.428) was determined for the species T. sajanensis and T. vicarious and the smallest distance ( D = 0.091) for T. altaicus and T. asiaticus . Based on ISSR profile all individuals of T. sajanensis have been treated as hybrids.
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