INDICATION OF THE VACCINIA VIRUS BY A NANOWIRE SILICON-ON-INSULATOR BIOSENSOR
V. M. Generalov1, O. V. Naumova2, S. A. Pyankov1, I. V. Kolosova1, A. S. Safatov1, B. N. Zaitsev1, E. G. Zaitseva2, G. A. Buryak1, A. A. Cheremiskina1, N. A. Filatova1, A. L. Aseev2
1Federal Budgetary Research Institution - State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being, Koltsovo, Novosibirsk region, Russia
2Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
Keywords: biosensor, silicon-on-insulator, nanowire, vaccinia virus, indication
Abstract
The results of the indication of the vaccinia virus by using nanowire biosensors made on the silicon-on-insulator basis are presented. In the experiments, we used the vaccinia virus, the strain of LIVP from the collection of the Federal State Scientific Research Center of Virology and Biotechnology "Vector" of Rospotrebnadzor, and the rabbit blood serum containing specific polyclonal antibodies to the vaccinia virus. The results show that the polyvalent blood serum at the interface between the biosensor surface and the viral suspension is electrically neutral, the vaccinia virus is positively charged, and the complex of the polyvalent blood serum and vaccinia virus is negatively charged.
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