Genetic polymorphism of Siberian stone pine (Pinus sibirica Du Tour) in Kuznetsk Alatau
N. V. Oreshkova1,2,3, T. S. Sedel’nikova3, S. P. Efremov3, A. V. Pimenov2,3
1FRC KSC SB RAS, Krasnoyarsk, Russia
2Siberian Federal University, Krasnoyarsk, Russia
3V. N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, Russia
Keywords: microsatellites, genetic diversity, heterozygosity, Kuznetsk Alatau
Abstract
The DNA polymorphism of 7 coenopopulations of Siberian stone pine ( Pinus sibirica Du Tour), growing in the region which is typical for mountain taiga pine forests - the north-eastern part of the Kuznetsk Alatau, was studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants, which significantly differed between the studied cenopopulations in composition and frequency of occurrence were identified. The highest level of allelic diversity is found in the Ps_80612, Ps_1502048 loci, in which 8 and 7 alleles were detected, respectively. The calculation of the main parameters of genetic diversity showed, on the whole, a relatively low level of polymorphism of the studied samples ( NA = 3,078; NE = 1,877; HE = 0,445; HO = 0,401). An analysis of the degree of subdivision of cenopopulations revealed that about 95 % of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5 % ( FST = 0,049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations which defined the directions of seed propagation vectors along the watercourses of river basins that formed the Chulym river in the eastern sector of the Kuznetsk Alatau and Tom river in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of cenopopulations of P. sibirica , morphological singularities and reproductive potential of trees and, ultimately, control their genetic polymorphism in the studied part of the Kuznetsk Alatau.
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