Content of matrix metalloproteinases in the intraocular fluid of patients with primary open-angle glaucoma
Valery V. Chernykh1, Vladimir I. Konenkov2, Nikolay B. Orlov2, Olga V. Ermakova1, Aleksandr N. Trunov3,4
1Novosibirsk Branch of the S. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk, Russia
rimma@mntk.nsk.ru
2Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Cytology and Genetics of SB RAS, Novosibirsk, Russia
vikonenkov@gmail.com
3Novosibirsk Branch of
the S. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk, Russia
trunov1963@yandex.ru
4Federal
Research Center for Fundamental and Translational Medicine
Keywords: первичная открытоугольная глаукома, матриксные металлопротеиназы, внутриглазная жидкость, primary open-angle glaucoma, matrix metalloproteinases, intraocular fluid
Abstract
Aim of the study was to
investigate the content of matrix metalloproteinases 2, 3, 9, in the
intraocular fluid of patients with a developed stage of primary
open-angle glaucoma. Material and methods. The study included 47
patients with advanced stage of primary open-angle glaucoma. The
diagnosis is based on ophthalmological examination, including visual
acuity, binocular ophthalmoscopy, spheroperimetry, echoophthalmography,
optical coherence tomography, intraocular pressure measurement.
Exclusion criteria were acute and exacerbation of chronic inflammatory
diseases of the organ of vision, diabetic retinopathy, neovascular
glaucoma, uveitis, hemophthalmia, autoimmune and tumor processes of any
localization, as well as acute and exacerbations of chronic inflammatory
diseases of any organs and systems. Patients who took for normalization
of intraocular pressure drugs containing analogues of prostaglandins,
that could activate the inflammatory process, were excluded from the
study. 26 patients with a diagnosis of uncomplicated cataract made up a
comparison group. All patients underwent determination of matrix
metalloproteinases (MMP) 2, 3, 9 in the intraocular fluid. Results.
Concentration of MMP-2 but not MMP-3 or MMP-9 is statistically
significantly higher in the intraocular fluid of patients with a
developed stage of primary open-angle glaucoma compared to individuals
with uncomplicated cataracts. This may indicate importance of MMP-2 in
the development of a local aseptic inflammatory process, impaired
extracellular matrix and «remodeling» of the tissue structures of the
organ of vision, leading to disruption of the outflow of intraocular
fluid, which is an important factor in the pathogenesis of primary
open-angle glaucoma.
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