Home – Home – Jornals – Siberian Scientific Medical Journal 2020 number 5

Aim of the study was to investigate the distribution features of HLA alleles and multilocus haplotypes in potential donors of hematopoietic stem cells recruting in the Sverdlovsk, Saratov, Yaroslavl and Vladimir regions. Material and methods. Sequence Based Typing technology was used to identify human leukocyte antigen (HLA)-A, -B, -C, -DRB1 alleles from 2683 Russian unrelated bone marrow volunteers living in the Sverdlovsk ( n = 1018), Saratov ( n = 825), Yaroslavl ( n = 604) and Vladimir ( n = 236) regions. HLA allele and haplotype frequencies were estimated via maximum-likelihood analysis from genotypic data through an expectation-maximization (EM) algorithm for unknown gametic phase. Results and discussion. In all studied populations, 16 HLA-A, 13 HLA-C, 13 HLA-DRB1 alleles were selected. In the locus HLA-B, 28 alleles were detected in the populations of the Sverdlovsk and Yaroslavl regions, 27alleles - in the Saratov region, 25 alleles - in the Vladimir. Seventeen alleles, HLA-A*02, HLA-A*03, HLA-A*01, HLA-A*24, HLA-B*07, HLA-B*35, HLA-С*07, HLA-С*06, HLA-С*04, HLA-С*03, HLA-С*12, HLA-DRB1*15, HLA-DRB1*07, HLA-DRB1*01, HLA-DRB1*13, HLA-DRB1*04, HLA-DRB1*11 exhibit frequencies over 10 %. The highest frequency extended haplotype in the all studied populations HLA-A*01-B*08-C*07-DRB1*03, was observed frequencies of 4,4 % - in the Sverdlovsk region, 3,2 % - in the Saratov region, 4,9 % - in the Yaroslavl region and 4,2 % - in the Vladimir region. Routine HLA typing allowed us to define four new HLA alleles in the populations of the Sverdlovsk and Saratov region.