Home – Home – Jornals – Contemporary Problems of Ecology 2019 number 4

The evaluation of the morphogenetic potential in in vitro culture of the valuable medicinal plant Rhodiola rosea from six natural habitats as a basis for the development of effective methods of reproduction and conservation of this rare species has been carried out. The influence of different quality seeds from different habitats on germination in vitro ; the dependence of the regenerative capacity of R. rosea shoots on the concentration and combination of 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA); the effect of shoot precultivation on media with growth regulators on the development parameters of regenerants on a hormone-free medium 1/2MS. The dependence of the germination of in vitro seeds of R. rosea on the habitat of samples and shelf life is shown. It was noted that the introduction of growth regulators into the nutrient medium led to an increase in the multiplication factor by 1.9-2.8 times and a decrease in the height of the shoots by 2.4-3.3 times. Variant number 2 from the Sakhalin region was characterized by the highest average rates of shoot height and breeding rate. For variant N 4 from the Kamchatka Territory, various morphogenic reactions in an in vitro culture have been noted: sprouting, callus formation, flowering of plants. For all the studied variants, 100 % rooting on 1/2MS medium is typical. For variants N 1 and 5, the positive effect of pre-cultivation of explants on media containing 1 mg/l BAP alone or in combination with NAA 1 mg/l is shown to obtain optimal indicators of rhizogenesis and the development of regenerants. Significant differences in the parameters of growth and development of explants in in vitro culture depending on the composition of the nutrient medium and habitat of R. rosea are shown.