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2019 year, number 2

HETEROGENEITY OF 2.3 MDa PLASMIDS IN Salmonella ENTERITIDIS STRAINS

Alexey Vladimirovich RAKOV, Felix Nikolaevich SHUBIN, Nataliya Anatolievna KUZNETSOVA, Alina Sergeevna SOLOVYEVA
Somov Institute of Epidemiology and Microbiology
Keywords: Salmonella enteritidis, плазмида p2.3, плазмидный анализ, полимеразная цепная реакция, Salmonella Enteritidis, plasmid p2.3, plasmid analysis, polymerase chain reaction

Abstract

The aim of the research was to study plasmids 2.3 MDa (3609 bp) and their distribution in Salmonella Enteritidis strains isolated from various environmental sources at different times on the territory of the Russian Federation. Material and methods. A PCR typing of 33 S. Enteritidis strains, isolated for the period from 1995 to 2017, containing plasmid 2.3 MDa described earlier in microbe strains has been carried out. Results and discussion. The plasmid was firstly sequenced by us. Its relatedness to plasmid in GenBank from the strain of S . Typhimurium CFSAN001921 described in the United States has been revealed. It has been established that plasmid p2.3 presents within 23 years in S . Enteritidis strains isolated from patients and food at administrative territories of majority of the Russian Federation subjects. In addition, it has been shown that plasmid p2.3 can be heterogeneous in nucleotide composition. Conclusion. Considering the fact that plasmid p2.3 was isolated from patients in the USA, and strains we investigated were from patients and food in Russia, it can be assumed that the distribution area of the plasmid has a transcontinental nature.