MORPHOGENESIS of Picea pungens Engelm. in vitro UNDER THE INFLUENCE OF THIDIAZURON
T. V. Zheleznichenko1, D. S. Muraseva2, V. V. Stasova3, T. I. Novikova2
1Central Siberian Botanical Garden, Russian Academy of Sciences, Siberian Branch
2Central Siberian Botanical Garden, Russian Academy of Sciences, Siberian Branch, Zolotodolinskaya str., 101, Novosibirsk, 630090, Russian Federation
3V. N. Sukachev Institute of Forest, Russian Academy of Science, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
Keywords: гистология, культура in vitro, органогенез, микропобеги, тидиазурон, ель голубая, histological analysis, in vitro culture, organogenesis, clonal micropropagation, thidiazuron, blue spruce
Abstract
Specific features of the blue spruce Picea pungens Engelm. regeneration from mature zygotic embryos in vitro culture under the influence of thidiazuron (TDZ) were studied. It was noted that under the influence of TDZ the explants cotyledons tissues were expanded, and active morphogenesis took place and meristemoids were formed in their epidermal layers. During the plant material subculturing, which was obtained within the first passage, in the hormone-free culture media, the asynchronous development of structures and the variety of morphogenetic pathways were observed: adventitious buds, microshoots and individual needles were formed at different stages of development. This TDZ influence on the regeneration of blue spruce was noted by us for the first time. The maximum microshoots formation with normal morphology was achieved by using a two-stage explants cultivation: induction of morphogenic processes in the ½ LV culture medium at the TDZ concentration of 0.5 μM during the first passage (28 days) followed by transfer of the obtained material to the ½ LV hormone-free medium. The number of microshoots per explant was (70.3 ± 7.5) pcs. With an increase in the TDZ concentration to 1 μM the number of regenerants per explant decreased 1.6 times and the increased duration of the first passage up to 35 days led to anomalies and inhibition of the microshoots development.
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