Features of Antioxidant Activity of Tissues of Reophil Zoobenthos Representatives Based on the Chemiluminescent Analysis
G. V. MAKARSKAYA1,2, A. V. ANDRIANOVA1,3, S. V. TARSKIKH2
1Institute of Computationаl Modeling, SB RAS, 660036, Krasnoyarsk, Akademgorodok, 50/44
2International scientific centre for organism extreme conditions research at Presidium of Krasnoyarsk science centre, SB RAS, 660036, Krasnoyarsk, Akademgorodok, 50/44
3Federal state budgetary scientific establishment Scientific Research Institute of Ecology of Fishery Reservoirs, 660097, Krasnoyarsk, Parizhskoi Kommuny str., 33
Keywords: р.Енисей, донные беспозвоночные, амфиподы, активные формы кислорода, антиоксидантная система, перекисное окисление липидов, хемилюминесцентный анализ, the Yenisei River, benthic invertebrates, amphipods, reactive oxygen species, antioxidative system, lipid peroxidation, chemiluminescent analysis
Abstract
The adaptation of living organisms to the environmental conditions is formed and manifested at different levels - from the molecular to the biocenotic. Nowadays, the metabolic antioxidant system (AOS) is considered to be the key part of adaptive processes, and its activation is regarded as a general response of biota to the negative impact of the environment. The usage of AOS components as biomarkers of stressful influence on hydrobionts has good prospects and is actively applied in the monitoring system of aquatic ecosystems. In this paper the kinetics of free radical formation in various zoobenthos representatives from the Yenisei River (amphipods, oligochaetes, leeches, chironomids) and in amphipods from the Us River (a small mountain river, the Yenisei tributary) is analyzed. Parameters of kinetics of radical formation were registered using the method of luminoldepended chemoluminescence under the initiation of oxidative stress with hydric dioxide in vitro in homogenates of hydrobionts tissues. It was revealed that in amphipodes, as opposed to other invertebrates, the process of antioxidative activity is characterized by two chemiluminescent flashes. What is more, interspecies and age-related differences in parameters of the kinetics of free radicals formation were noticed in amphipodes. In Gmelinoides fasciatus Stebb. the intension peak of the formation of free radicals and their total number during the 60-minute registration period authentically exceeded these parameters for Gammarus sp. and Eulimnogammarus viridis Dybowsky. With age, the increase in volume of production of free radicals in E. viridis and Gammarus sp. and its decrease in G. fasciatus were observed. The authentically higher value of volume of production of free radicals in G. fasciatus and E. viridis inhabiting the zone of the Yenisei River with heightened anthropogenic load was marked.
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