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Chemistry for Sustainable Development

2015 year, number 5

Identification of the Components of Essential Oil of Pelargonium (Pelargonium graveolens L’Her) by Means of Thin Layer Chromatography

1Perm State Pharmaceutical Academy, Ul. Krupskoy 46, Perm 617070 (Russia)
2Nutrition Institute, Ministry of Power Engineering and Industry, Republic of Tadzhikistan, Ul. Tursun-Zade 25, Dushanbe 734025 (Tadzhikistan)
Keywords: эфирное масло герани душистой, эфирное масло, ТСХ, essential oil of Pelargonium graveolens L’Her, essential oil, TLC


A procedure based on thin layer chromatography (TLC) was proposed for the determination of the components of essential oil of Pelargonium graveolens L’Her manufactured in Tadzhikistan. Citronellol, geraniol, linalool, phenyl ethyl alcohol were chosen as the standard samples of witness substances (SSWS). As a result of the work, the following chromatography conditions were determined: eluting system - benzene/chloroform (1 : 1), detecting reagent - 5 % ethanolic solution of phosphomolybdic acid, volume of sample applied - 5 mL, concentration 0.005 g/ml. It was established that the polarioty of eluent affects the separation of the components of essential oil from Pelargonium graveolens L’Her. The optimal value of system polarity is 3.19. Seven spots are observed in the chromatograms of pelargonium essential oil manufactured in Tadzhikistan; four of these spots were identified on the basis of R f value and the positions of SSWS spots. The results showed that the chromatographic profile of essential oil from the plant species under under study differs from the essential oil of another manufacturer, essential oil with similar composition (citronellla oil and lemongrass oil) and widespread readily available oil (lavender and sage). Chromatographic profiles differ by the presence of additional spots with different number, size and position. The data obtained provide evidence of the possibility to use TLC as a rapid method to identify essential oil of pelargonium manufactured in Tadzhikistan.