THE USE OF GENETIC MARKERS OF VARIOUS TYPES FOR EVALUATION OF INTRASPECIFIC DIFFERENTIATION LEVEL OF THE SIBERIAN SPRUCE
A. K. Ekart1, S. A. Semerikova2, V. L. Semerikov2, A. N. Kravchenko1, O. S. Dymshakova2, A. Ya. Larionova1
1V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
2Institute of Plant and Animal Ecology, Russian Academy of Sciences, Ural Branch, 8 Marta str., 202, Yekaterinburg, 620144 Russian Federation
Keywords: Siberian spruce (Picea obovata Ledeb.), isoenzymes, nuclear and chloroplast microsatellites, genetic diversity, differentiation
Abstract
On the basis of the analysis of variability of isoenzyme loci and microsatellite loci of nuclear and chloroplast DNA the data were obtained about the degree of differentiation of a number populations of Siberian spruce (Picea obovata Ledeb.), including isolated populations located on the island of Olkhon, in the reserve Bogd–Uul (Mongolia) and on the south of the Magadan region. It was shown that the combined use of various types of genetic markers gives an opportunity to get more complete and objective information about a genetic diversity and intraspecific differentiation of this widespread in Siberia species.
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