STAGE–BY–STAGE CERTIFICATION OF TREES IN PLANTATIONS OF SCOTS PINE GENETIC BREEDING COMPLEX
V. V. Tarakanov1, L. I. Kalchenko2, K. G. Zatcsepina1,2, A. K. Ekart1, D. N. Shuvaev2
1V. N. Sukachev Institute of Forest Russian Academy of Sciences, Siberian Branch, Novosibirsk, Post Box 45, 630082 Russian Federation
2Centre for Forest Protection of Altai Territory, Proletarskaya str., 61, Barnaul, 656056 Russian Federation
Keywords: genetic certification, clone plantations, Scots pine (Pinus sylvestris L.), phens, allozyme analysis, Altai
Abstract
In Altai territory in Scots pine (Pinus sylvestris L.) clonal plantations, that include nearly 1.5 thousand trees of 118 clones, the stage-by-stage certification of trees was carried out. Certification process included 3 stages: 1) forestry stage (preliminary), 2) phenetic stage (basic), 3) genetical stage (précising). Accuracy of phenetic certification was 97.1 %. The final stage of certification was carried out taking into account of revealed «phenetic clusters» within of clones. One-ramet and many-ramets («mixed») samples were compared within clusters and these must show identical results if all ramets are truly marked. The stage-by-stage certification method allows less expensive molecular genetics testing in some cases.
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