CYTOLOGICAL, MOLECULAR-GENETIC AND SILVICULTURAL-SELECTION RESEARCH OF POLYPLOID POPLARS
A. I. Sivolapov1, D. V. Politov2, O. S. Mashkina3, M. M. Belokon2, V. A. Sivolapov4, Y. S. Belokon2, T. M. Tabatskaya5
1Voronezh State Academy of Forestry and Technology, Timiryazev str., 8, Voronezh, 394087 Russian Federation
2N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin str., 3, Moscow, 119991 Russian Federation
3Voronezh State University, Universitetskaya Ploschad’, 1, Voronezh, 394006 Russian Federation
4Centre for Forest Protection of Voronezh Region, Lomonosov str., 105, Voronezh, 394087 Russian Federation
5All-Russian Scientific and Research Institute for Forest Genetics, Breeding and Biotechnology, Lomonosov str., 105, Voronezh, 394087 Russian Federation
Keywords: poplar, aspen, polyploids, the number of chromosomes, DNA analysis, microsatellite loci, genetic passportization, productivity of poplar cultures
Abstract
The results of cytological, silvicultural and selection research and DNA analysis of polyploid poplars are discussed in the article. Poplar clones were studied for productivity, the number of chromosomes, selection and testing of microsatellite loci for genetic certification of planting material. The study showed a high diagnostic performance of species, of individual genotypes and clones within species and hybrids on the basis of gene markers of polymorphism of microsatellite DNA.
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