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Atmospheric and Oceanic Optics

2014 year, number 6

Insecticidal, antimicrobial, and antiviral activities of Bacillus thuringiensis ssp. kurstaki strains isolated from atmospheric aerosols of Southwestern Siberia

I.S. Andreeva1, A.S. Safatov1, O.S. Mokrushina1, G.A. Buryak1, L.I. Puchkova1, N.A. Mazurkova1, L.I. Burtseva2, G.V. Kalmykova3
1State Research Center of Virology and Biotechnology "Vector", Novosibirsk region, Koltsovo, 630559, Russia
2Institute of Systematics and Ecology of Animals of the Siberian Branch of the RAS, 930091, Novosibirsk, 11, Frunze str
3Siberian Research Institute pererabotki sel'skohozjajstvennoj produkcii Rossel'hozakademii, Novosibirsk region, Krasnoobsk, 630501, Russia
Keywords: atmospheric aerosols, Bacillus thuringiensis, insecticidal, antimicrobial, antiviral activity


The article presents data on insecticidal, antimicrobial, and antiviral activities of Bacillus thuringiensis (Bt) strains, isolated during microbiological analysis of high-altitude and ground-based samples of atmospheric aerosol collected in Southwestern Siberia. According to the results of intraspecies tests, among 36 isolated bacteria identified as belonging to Bt species, 15 strains were classified as Bt ssp. kurstaki subspecies and tested for the presence of antagonistic characteristics. Despite their long-term soaring under extreme conditions as a part of atmospheric aerosols, including high-altitude ones (up to 7000 m), the isolated Bt ssp. kurstaki strains preserved high levels of entomopathogenic activity characteristic for this subspecies, were able to inhibit the reproduction of pathogens such as Staphylococcus aureus, Salmonella thyphimurium, Shigella sonnei, Candida albicans, etc. and to effectively neutralize the replication of avian influenza virus A/chicken/Kurgan /05/2005 (A/H5N1) and human influenza virus A/Aichi/2/68 (A/H3N2). The properties of Bt strains isolated from atmospheric aerosols not only confirmed their well-known resistance to adverse environmental factors, entomopathogenic and antimicrobial activities, but also demonstrated the possibility of their use for antiviral drugs development.