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Contemporary Problems of Ecology

2013 year, number 6

Characterization of the Communities of Microorganisms from Lake Baikal Developing on a Steel Plate and in Natural Water Surrounding This Substrate

V. V. MALNIK1, V. V. PARFENOVA1, A. N. SUTURIN1, O. A. TIMOSHKIN1, N. S. PAVLOVSKAYA2
1Limnological Institute, SB RAS, 664033, Irkutsk, Ulan-Batorskaya str., 3
2Siberian Institute of Physiology and Biochemistry of Plants, SB RAS, 664033, Irkutsk, Lermontov str., 132
Keywords: biofilm, Lake Baikal, microorganisms, 16S rRNA, RNA-polymerase, rpoC gene, cloning

Abstract

Analysis of the microbial community developing on a plate made of stainless steel is presented. The sample was analyzed for the rpoC gene from this plate. Identification of dominant species (clones) of the biofilm was performed using molecular cloning and determination of the nucleotide sequence of the region of 16S rRNA gene. A comparison between the activities of the microorganisms of the plankton way of life and those adherent in the biofilm is presented. It was discovered that 7 of 19 treated sequences (37 %) belonged to the Sphingobacteria group. Analysis of nucleotide sequences of the 16S rRNA gene, cloned from natural biofilm samples from Lake Baikal, revealed a broad variety of microorganisms incorporated in the biofilm. The following taxonomic groups were revealed among them: sphingobacteria, α-proteobacteria, cyanobacteria, verrucomicrobia, bacilli, γ-proteobacteria, flavobacteria and β-proteobacteria. The analysis of this kind has been performed for Lake Baikal for the first time.