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Contemporary Problems of Ecology

2013 year, number 4

Callus Formation of Ecgysteroid Species Silene L. (Caryophyllaceae Juss.) in Culture in vitro

A. A. ERST1, L. N. ZIBAREVA2
1FSIS Central Siberian Botanical Garden SB RAS, 630090, Novosibirsk, Zolotodolinskaya st., 101
annаerst@yandex.ru
2Siberian Botanical Garden of TSU, 634050, Tomsk, pr. Leninа, 36
zibareval@inbox.ru
Keywords: cell culture, Silene, ecdysteroids, growth index

Abstract

Continuously growing callus cultures of three species of Silene genus – S. linicola, S. aprica and S. frivaldszkyana were obtained. It was shown that the genotype and type of explant influence the processes of callus. The highest growth indexes had calluses from hypocotyl – 10,1 ± 1,4, 11,6 ± 3,4 and 7,4 ± 1,1 ( S. linicola, S. aprica and S. frivaldszkyana respectively).