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Contemporary Problems of Ecology

2005 year, number 4

Interspecific and Intraspecific Differentiation of Siberian Larch Species Using the in vitro Cell Culture Technique

Yu. M. KONSTANTINOV, V. N. SHMAKOV

Abstract

Possibility of differentiation of two Siberian larch species (Larix sibirica and L. gmelinii) at species and . population levels using long-living cell cultures in vitro was studied. For the first time interspecific and intraspecific differences in proliferative activity of calluses were found in Siberian larch species on a medium with sodium fluoride were found. A conclusion was made that larch callus culture can be used effectively for the studies of inter-and intraspecific polymorphism in larch with respect to sensitivity to fluorine and other technogenous pollutants when studying possible genetic consequences of action of industrial emissions on cell genome of larch.