STUDY ON THE INTERACTION OF A PALLADIUM COMPLEX WITH DNA
E.J. Gao, H.X. Yin, M.C. Zhu, Y.G. Sun, X.F. Gu, Q. Wu, L.X. Ren
Keywords: palladium(II) complex, adenine, interaction mechanism, kinetics, crystal structure
Pages: 1086-1091
Abstract
The complex [Pd(bipy)Cl2] (1) (bipy = 2,2′-bipyridyl) has been synthesized and characterized by NMR spectroscopy, elemental analysis and X-ray diffraction method. The first step hydrolysis reaction kinetics for the complex was studied by UV-absorption spectroscopy; the speed constant (k1) was found to be 3.0×10-4 s-1. The fluorescence spectra have been collected to investigate the interaction of complex (1) with fish sperm DNA (FS-DNA) and the results indicate that the complex (1) has an effective intercalation within DNA. The reaction of complex (1) with adenine in ethanol/water results in the compound [Pd2(bipy)2(ade)2]Cl2⋅3H2O (2) (ade = adenine) whose crystal structure was determined by X-ray diffraction method. The structure is orthorhombic, Pmmn, a = 12.993(4), b = 14.512(5), c = 9.837(3) Å, V =
1854.8(11) Å3, Z = 2 (C30H30Cl2N14O3Pd2), final R1 = 0.0675. The palladium complex is a binuclear cation, where two ade ligands bridge two Pd(II) centers, while each Pd(II) is also chelated by one bipy ligand.
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