Chemical fingerprinting for the study of living systems
S. V. MOROZOV1,2, E. I. CHERNYAK1, T. G. PCHELNIKOVA1, A. YU. LOPATKOV1, M. D. GANINA1, N. I. TKACHEVA1
1Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
2Novosibirsk State University, Novosibirsk, Russia
Keywords: chemical fingerprinting, gas chromatography, mass spectrometry, characteristic target metabolites, chromatographic profiling, living systems, biologically active substances
Abstract
Results obtained in the studies of living systems using chemical fingerprinting methodology are presented. Gas chromatography - mass spectrometry and the tools of chemical fingerprinting were applied to obtain the chromatographic profiles and to determine the isomeric-homologous series of characteristic target metabolites of various classes of the objects of plant origin and cuticular lipids of insects at different stages of ontogenesis and living in different climatic conditions. The main target metabolites considered herein are hydrocarbons, ketones, alcohols, fatty acids and their hydroxy-derivatives, esters, alkylresorcinols, di- and triterpenoids, phenol acids, and lignans. The approach used in the work makes it possible to establish the composition of characteristic target metabolites in various living systems and their changes in the processes occurring under the influence of abiotic and biotic factors, targeted gene mutations, which can be used both in fundamental and applied research, including the improvement of approaches to biological control of economically important insect pests in agriculture.
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