Assessment of the ecological state of the Azov Sea waters using biochemical parameters in the liver of round goby Neogobius melanostomus (Pallas, 1814)
T. B. SIGACHEVA, E. N. SKURATOVSKAYA
A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia
Keywords: round goby, liver, biochemical parameters, complex pollution, Sea of Azov
Abstract
The biochemical parameters (albumin content, levels of oxidized proteins (OP) and lipid peroxidation (LPO), antioxidant enzyme activities (superoxide dismutase (SOD) and catalase), aminotransferases and cholinesterase activities were analyzed in the liver of round goby Neogobius melanostomus from the Sea of Azov areas (central (CP), southern (SP) and southeastern (SEP)) with different anthropogenic impacts. Intensification of LPO and protein oxidation processes, as well as protein metabolism reorganization towards gluconeogenesis and detoxification reactions found in the liver of gobies from SEP have been established. This may indicate a more environmentally unfavorable condition of SEP as compared to SP and CP. The analysis of biochemical parameters in the liver of fish from SP and CP allowed characterizing these zones as similar by the level of mixed pollution, however more obvious changes in the biochemical parameters (inhibition of SOD activity, increase in the level of protein oxidation) were observed in the liver of gobies from SP. The obtained results permitted to rank the investigated areas according to the increase the level of mixed pollution as follows: CP → SP → SEP. Suggested reasons for the identified changes were discussed.
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