Interannual variability of low molecular weight metabolome of Nuphar lutea (L.) Sm. (Nymphaeaceae) in Lake Kuznechnoe (Leningrad region) under different trophic states
V. V. ANIKINA1,2, E. YA. YAVID3, Yu. V. KRYLOVA4, E. A. KURASHOV4, V. A. GREBENNIKOV1, E. V. PROTOPOPOVA2, P. V. BASH2
1Federal state budgetary scientific institution "Russian Federal Research Institute of Fisheries and Oceanography", St. Petersburg Branch, St. Petersburg, Russia
2St. Petersburg Federal Research Center of the Russian Academy of Sciences, St. Petersburg, Russia
3GazEnergoStroy LLC, Novy Urengoy, Russia
4Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok settlement, Russia
Keywords: low molecular weight metabolome, component composition, low-molecular weight organic compounds, gas chromatography-mass spectrometry, Nuphar lutea, cyanobacteria, allelochemicals, Kuznechnoye Lake, trophic status, indication of ecological status
Abstract
For the first time, a chromatograph mass spectrometric study of the essential oil of the yellow water lily (Nuphar lutea (L.) Sm.) growing in Lake Kuznechnoye in the Leningrad Region on the Karelian Isthmus was carried out at the peak of plant development in the flowering-fruiting phase in 2020 and 2021. The aim is to study the interannual variability of the component composition of low-molecular organic compounds (LMWOC) and assess the possibility of using its low-molecular metabolome (LMWM) to indicate the ecological state of water bodies. The limnological characteristics of the reservoir in the place where the yellow water lily grows are given. The main research method of the work is gas chromatograph mass spectrometry (GC-MS). It was revealed that under changing environmental conditions in different years, including depending on a specific biotic environment (phytoplankton) and abiotic factors, plants are able to synthesize different numbers of LMWOC. The decrease in the trophic status of the lake ecosystem and the decrease in the development of cyanobacteria, as the dominant component of phytoplankton, leads to a significant increase in the content of all groups of components in the LMWM of the yellow water lily. Intraspecific interannual variability of the LMWM of the yellow water lily can serve as an indicator of stability/changes in the state of the aquatic ecosystem. High quantitative indicators of the content of LMWOC, especially allelochemical substances, identified in N. lutea from Lake Kuznechnoye, typical for the Karelian Isthmus, allow us to consider the yellow water lily growing in the lakes of the Karelian Isthmus as an underutilized natural resource of valuable LMWOC, which has the potential for diverse economic use.
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