REPRODUCTION OF KOREAN POPLAR in vitro
T. P. Orekhova
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
Keywords: Populus koreana Rehder, explant, media, hormones, meristem, shoot, callus, rhizogenesis
Abstract
The patented method for introducing in vitro culture and obtaining regenerates from young shoots of Korean poplar ( Populus koreana Rehder) plus tree of the male genotype are discussed in the article. Modified MS and ½ MS media supplemented with an antibiotic and hormones were used for cultivation. To activate the axillary meristem, a combination of hormones was used in the following concentrations: 0.2 mg/l BA; 0.05-0.1 mg/l TDZ; 0.01 mg/l NAA. The shoots were multiplied on the same media using hormones: 0.4-0.6 mg/l BA and 0.1 mg/l IBA. Shoot growth was performed using the hormone 0.1 mg/l kinetin, and shoot rhizogenesis in the presence of 0.25-0.5 mg/l IBA and 0.1 mg/l kinetin.The sterile substrate consisting of equal parts of sand, vermiculite and forest soil was used for plants rooted. Poplar regenerants are differed in growth rate, but no morphological changes among them were observed. Some of the plants are characterized by rapid growth in height, while other plants developed very slowly. The tallest plant specimens in clones 1/1, 1/8 and 1/14 were observed, and their height varied from 18 to 30 cm. We believe that the appearance of plants with different growth rates is the result of the manifestation of somaclonal variability poplar during its cultivation in vitro . However, this phenomenon requires further confirmation. The plants obtained by this method do not have fungal, bacterial or viral infections and are able to reproduce by cuttings. The proposed technology enables for their further reproduction poplar for practical purposes.
|
