Variability of morphometric characteristics and genetic differentiation according to ISSR markers in Adonis villosa Ledeb. (Ranunculaceae) in the Altai Republic
E. V. ZHMUD1, I. N. KUBAN1, A. A. ACHIMOVA2, O. N. PAPINA3, O. V. DOROGINA1,4
a:2:{s:4:"TEXT";s:330:"1Central Siberian Botanical Garden of SB RAS, Novosibirsk, Russia
2Central Siberian Botanical Garden of SB RAS, Altai Branch “Gorno-Altai Botanical Garden”, Kamlak, Russia
3Gorno-Altaisk State University, Gorno-Altaisk, Russia
4Novosibirsk State University, Novosibirsk, Russia";s:4:"TYPE";s:4:"html";}
Keywords: fluffy Adonis, fluffy Starodubka, vulnerable species, cenopopulations, genetic polymorphism, parameters of genetic diversity
Abstract
The abundance and morphogenetic indicators of the Adonis villosa Ledeb., a vulnerable species in the Altai Republic (AR), have been studied. For the first time, genetic differentiation in six cenopopulations (CP) of A. villosa using ISSR markers and variability of morphometric characters in individuals from four CPs in the AR were revealed. Similarities in the variability of morphometric characters in the studied CPs were revealed, as well as a decrease in the number of mature generative individuals by 1.5-14.0 times over the period from 2017 to 2023. The genetic structure was studied using 6 primers: M 9, UBC 834, UBC 830, UBC 857, UBC 840, UBC 811. Of these, UBC-857 turned out to be the most informative. In representatives of the six studied CPs, we identified four variants in the distribution of ISSR markers, and a high similarity in the distribution of ISSR markers between the A. villosa CPs (88-99 %) was found. Intrapopulation similarity among individuals of this species is also high and reached 97 %. The probable reasons for this phenomenon are the small number of mature generative individuals in the CP (no more than 10) and their spatial isolation. In Northern Altai, A. villosa individuals have a homogeneous genetic structure, which leads to the vulnerability of this species. This species requires a special approach to conservation in natural conditions and culture.
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