PHYTOCHEMICAL CHARACTERIZATION AND ANTIVIRAL ACTIVITY OF ASTRAGALUS GLYCYPHYLLOS (FABACEAE) EXTRACTS
Irina E. Lobanova1, Tatiana A. Kukushkina1, Tatiana M. Shaldaeva1, Ekaterina I. Filippova2, Maria A. Protsenko2, Elena V. Makarevich2, Natalya A. Mazurkova2, Elena P. Khramova1
a:2:{s:4:"TYPE";s:4:"HTML";s:4:"TEXT";s:189:"1Central Siberian Botanical Garden, SB RAS, Novosibirsk, Russia
2State Scientific Center of Virology and Biotechnology “Vector” of Rospotrebnadzor, Koltsovo, Russia";}
Keywords: Fabaceae Astragalus glycyphyllos, biologically active substances, antiradical and antiviral activity, influenza A virus
Abstract
Comparative data of phytochemical evaluation of the content of biologically active substances, antiradical activity and antiviral properties in plant raw materials of Astragalus glycyphyllos L. from wild populations of the forest-steppe zone of Western Siberia are presented. During the vegetation period the dynamics of the content and the peculiarities of accumulation of the total content of phenolic compounds, flavonoids, tannins, phenolic acids, saponins, carotenoids, chlorophylls a and b, pectins and protopectins were investigated, changes in antiradical activity and antiviral action against human influenza virus - A/Aichi/2/68 (H3N2) and bird - A/chicken/Kurgan/05/2005 (H5N1) in extracts obtained from plant raw materials of above-ground and underground parts of A. glycyphyllos. It was found that the aboveground part of A. glycyphyllos is richer in BAS content than its underground part. In the aboveground part of A. glycyphyllos maximum amounts of most biologically active substances of the studied groups are accumulated in different phases of development, but mainly in the phase of fruiting termination. In the underground part, only protopectins were found in maximum amounts in the phases of the beginning of vegetation and fruiting. The antiradical activity of extracts from above-ground and underground parts of A. glycyphyllos was manifested during the whole vegetation period, but samples from raw materials of above-ground and underground parts at the end of vegetation were more effective. The study of toxicity and antiviral effect of extracts obtained from raw materials of both parts of Astragalus glycyphyllos in maximum tolerated concentrations showed that the IN of the extracts was within the range from 0 to 1.5 lg, depending on the subtype of influenza A virus, the growing season of A. glycyphyllos, its part in the study and the type of extract. Compared to the control, there was a significant decrease in the infectivity of human influenza virus and avian influenza virus under the influence of A. glycyphyllos extracts.
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