MILLIFLUID CUVETTE FORMED BY PHOTOPOLYMER STEREOLITHOGRAPHY TO MEASURE RAMAN SCATTERING FROM SINGLE OOCYTES
K. A. Okotrub1, Yu.V. Zaitseva1, S.V. Adichtchev1, T. A. Rakhmanova1,2, S.Y. Amstislavsky1,2
1Institue of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
2Institue of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
Keywords: Raman scattering, biological cell, microfluidic device, stereolithography
Abstract
Here we propose the use of photopolymer stereolithography to create millifluidic devices for spectroscopic studies. The efficiency of the approach is demonstrated by an example of the development of a millifluid cuvette for studying single mouse oocytes using Raman scattering under conditions of a varying composition of the surrounding solution. It is shown that using the “sandwich” cuvette designallows Raman measurements from samples inside the cuvette, despite the intense signal from the material from which the cuvette is made.
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