Investigation of the Chromatographic Behavior of Metabolites from Blood Plasma by HPLC-MS/MS Using a Monolithic Column with a Sorbent Based on 1-Vinyl-1,2,4-triazole
N. V. BASOV1, A. D. ROGACHEV1,2, YU. S. SOTNIKOVA1,2,3, YU. V. PATRUSHEV1,3, A. G. POKROVSKY1
1Novosibirsk State University, Novosibirsk, Russia
2Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
3Boreskov Institute of Catalysis, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
Keywords: 1-vinyl-1,2,4-triazole, monolithic columns, high performance liquid chromatography, mass spectrometry, chromatography of hydrophilic interactions, reversed-phase chromatography, metabolomics
Pages: 571-578
Abstract
The chromatographic behavior of a number of human blood plasma metabolites in HPLC-MS/MS analysis using a monolithic chromatographic column with a 1-vinyl-1,2,4-triazole-based sorbent was studied. Acetonitrile and a buffer based on ammonium carbonate and ammonia (pH 9.8) were used as the mobile phase. The experiment was carried out in two chromatographic modes: reversed-phase and hydrophilic. The metabolites selected for analysis allow for an unambiguous demonstration of the behaviour of both polar molecules and hydrophobic or amphiphilic lipid molecules in both of the chromatographic modes used. It was discovered that the change of chromatographic regime was accompanied by an inversion of the order of elution of metabolite groups having differences in chemical structure and hydrophilicity. It was shown that this 1-vinyl-1,2,4-triazole-based monolithic column can be used to screen metabolites in both reverse-phase and hydrophilic chromatography modes, and the use of two chromatography modes in metabolome screening using this column is mutually complementary and can help to increase the coverage of metabolites from different metabolic pathways.
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