CONSERVATION AND PROPAGATION OF
RHODODENDRON SCHLIPPENBACHII USING BIOTECHNOLOGICAL METHODS
Yu.G. Zaytseva, T.I. Novikova
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
ulianna_zaitseva@mail.ru
Keywords: Rhododendron schlippenbachii, размножение in vitro, зеатин, тидиазурон, Rhododendron schlippenbachii, in vitro propagation, zeatin, thidiazuron
Abstract
A protocol for R. schlippenbachii micropropagation from seeds revealed the high germinating capacity and shallow type of dormancy was developed. Th e effect of cytokinins such as zeatin and thidiazuron (TDZ) on morphogenic potential of R. schlippenbachii seedling was tested. The highest regeneration rate (100 %) was achieved in the presence of 1.0 μM TDZ or 2.5 μM of zeatin in Anderson’s nutrient medium (AM). Th e direct organogenesis occurred both through activation of axillary meristems and differentiation of adventitious buds on explants hypocotyl. The maximum number of shoots per explant was obtained under 1.0 μM TDZ. Th e cultivation of regenerants on hormone-free AM allowed to obtain the elongated shoots and to overcome the anomalies caused by TDZ treatment. The largest percentage of rooting plants was achieved after pulse treatment with 148.0 μM indolyl-3-butyric acid for 4 hours followed by transferring to ex vitro conditions in mixture of peat and sand. Th e results could be included in the program for conservation of this rare polymorphic ornamental species and creation of new varieties on its basis.
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