VALIDATION OF HETEROPHASE ANALYSIS OF RNA USING A SOI-FET BIOSENSOR
E. V. Dmitrienko1,2, A. V. Poryvaeva1, O. V. Naumova3, B. I. Fomin3, M. S. Kupryushkin1, I. A. Pyshnaya1, D. V. Pyshnyi1,2
1Institute of Chemical Biology & Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
2Novosibirsk State University, Novosibirsk, Russia
3A. V. Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
Keywords: SOI-FET biosensor, Si/SiO surfaces, heterophase analysis, hybridization, electrically neutral phosphoryl guanidine oligonucleotides
Abstract
The analysis of nucleic acids remains an important trend in the development of medical diagnostics. Modern ultrasensitive diagnostic systems make it possible to convert a specific interaction during the analysis into a hardware-recorded signal. An example of such diagnostic devices is biosensors based on silicon-on-insulator (SOI) field-effect transistors (FET). In this work, we use glass surfaces (as a Si/SiO2 surface of a SOI biosensor) to optimize and test all stages of the analysis. Efficient immobilization of electrically neutral analogs of oligonucleotides on the Si/SiO2 surface after activation of Si-OH groups with 3-glycidoxypropyltrimethoxysilane or carbonyldiimidazole is demonstrated. The possibility of detecting an RNA target on model glass surfaces in the parallel analysis mode under conditions of a low or zero salt concentration is provided. The regeneration of the Si/SiO2 surface of the sensors for the repeated analysis and the stability of the sensors during long-term storage are demonstrated.
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