ANALYSIS OF HAPLOTYPES OF CAT, TLR4, AND IL10 GENES IN BRONCHIAL ASTHMA PATIENTS COMORBID WITH ARTERIAL HYPERTENSION
Elena Yurjevna BRAGINA1, Irina Alexandrovna GONCHAROVA1, Maxim Borisovich FREIDIN1, Irina Zhargalovna ZHALSANOVA1, Densema Evgenjevna GOMBOEVA1, Evgeniy Vladimirovich NEMEROV2, Valeriy Pavlovich PUZYREV3,2
1Research Institute of Medical Genetics of Tomsk National Research Medical Center of RAS
2Siberian State Medical University of Minzdrav of Russia
3Research Institute of
Medical Genetics of Tomsk National Research Medical Center of RAS
Keywords: бронхиальная астма, артериальная гипертензия, коморбидность, полиморфизм генов, гаплотип, CAT, TLR4, IL10, bronchial asthma, hypertension, comorbidity, polymorphism of genes, haplotype, CAT, TLR4, IL10
Abstract
Co-occurrence of
cardiovascular diseases is significantly common among patients with
bronchial asthma. Genetic factors can have a significant effect on the
development of hypertension in patients with asthma. Objective of the
study was to investigate the associations of polymorphic variants
relating to quantitative changes in the expression profile (eQTL) of the
CAT , TLR4 , and IL10 genes with the development of bronchial asthma
co-morbid with arterial hypertension. Material and methods. Genotyping
of 48 eQTL SNPs of the CAT, TLR4 , and IL10 genes was performed using
MALDI-TOF mass spectrometry in patients with «isolated» asthma ( n =
145) and arterial hypertension ( n = 144) and their combination ( n =
146), as well as in the control group of healthy individuals ( n = 152).
Using logistic regression, an analysis of the associations of
haplotypes with the studied diseases was carried out. Results. An
association of bronchial asthma in combination with arterial
hypertension with haplotypes formed by eQTL SNPs of the CAT and TLR4
genes was established. The spectrum of haplotypes associated with
comorbidity of asthma and hypertension differs from the haplotypes
associated with “isolated” asthma. Conclusion. The molecular base of
asthma and hypertension comorbidity can be associated with variants that
control the expression of TLR4 and CAT genes.
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