ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met
a:2:{s:4:"TEXT";s:232:"Aleksandr Yuryevich ZHARIKOV, Nikolay Nikolaevich YAKUSHEV, Ganna Viktorovna ZHARIKOVA, Olesya Nikolaevna MAZKO, Olesya Gennadyevna MAKAROVA, Valeriy Mikhaylovich BRYUKHANOV, Yuriy Vladimirovich KORENOVSKIY, Olga Vasil’yevna AZAROVA";s:4:"TYPE";s:4:"text";}
Altai State Medical University of Minzdrav of Russia
Keywords: мочекаменная болезнь, тетрапептид, окислительный стресс, urolithiasis, tetrapeptide, oxidative stress
Abstract
The aim of the study was to
investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity
of free radical oxidation (FRO) and the level of cyclooxygenase-1
(COX-1) and COX-2 expression in rat kidney with experimental oxalate
nephrolithiasis. Material and methods. The experiments were carried out
on 30 male rats Wistar runoff, divided into 3 groups: the comparison
group (10 intact rats), the control group (6 weeks of experimental
oxalate nephrolithiasis simulation, n = 10), the experimental group (6
weeks of experimental simulation oxalate nephrolithiasis + oral
administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg
from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was
simulated according to conventional ethylene glycol model. COX-1 and
COX-2 concentration in rat urine at baseline and after 6 weeks of
experimental nephrolithiasis was determined by enzyme immunoassay using
the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from
«Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators
of FRO processes were determined by common methods. Results and
discussion. At the background of the Leu-Ile-Lys-Met tetrapeptide, free
radical kidney damage was weakened, which was manifested in the complete
absence of morphological signs of nephrolithiasis, decrease of
thiobarbituric acid-reactive product concentration and overall
prooxidant activity as compared to the control group; glutathione
peroxidase activity, on the contrary, increased by 1.5 times.
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