ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met
Aleksandr Yuryevich ZHARIKOV, Nikolay Nikolaevich YAKUSHEV, Ganna Viktorovna ZHARIKOVA, Olesya Nikolaevna MAZKO, Olesya Gennadyevna MAKAROVA, Valeriy Mikhaylovich BRYUKHANOV, Yuriy Vladimirovich KORENOVSKIY, Olga Vasil’yevna AZAROVA
Altai State Medical University of Minzdrav of Russia
Keywords: мочекаменная болезнь, тетрапептид, окислительный стресс, urolithiasis, tetrapeptide, oxidative stress
Abstract
The aim of the study was to
investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity
of free radical oxidation (FRO) and the level of cyclooxygenase-1
(COX-1) and COX-2 expression in rat kidney with experimental oxalate
nephrolithiasis. Material and methods. The experiments were carried out
on 30 male rats Wistar runoff, divided into 3 groups: the comparison
group (10 intact rats), the control group (6 weeks of experimental
oxalate nephrolithiasis simulation, n = 10), the experimental group (6
weeks of experimental simulation oxalate nephrolithiasis + oral
administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg
from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was
simulated according to conventional ethylene glycol model. COX-1 and
COX-2 concentration in rat urine at baseline and after 6 weeks of
experimental nephrolithiasis was determined by enzyme immunoassay using
the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from
«Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators
of FRO processes were determined by common methods. Results and
discussion. At the background of the Leu-Ile-Lys-Met tetrapeptide, free
radical kidney damage was weakened, which was manifested in the complete
absence of morphological signs of nephrolithiasis, decrease of
thiobarbituric acid-reactive product concentration and overall
prooxidant activity as compared to the control group; glutathione
peroxidase activity, on the contrary, increased by 1.5 times.
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