GENDER FEATURES OF LAMINA CONTENT IN HUMAN SKIN FIBROBLASTS IN A PROCESS OF CHRONOLOGICAL AGING
Natalya Nikolaevna Golubtsova, Aleksey Vladimirovich Bogdanov, Andrey Germanovich Gunin
Chuvash State University n.a. I.N. Ul’yanov
Keywords: ламин А, ламин В1, ламин В2, фибробласты, кожа, гендерные особенности, хронологическое старение, lamin A, lamin B1, lamin B2, fibroblasts, skin, gender features, chronological aging
Abstract
The purpose of the study
was to investigate gender peculiarities of the content of fibroblasts
immunopositive to lamins A, B1 and B2 in skin samples in a process of
chronological aging. Material and methods. 331 skin samples at the
period from 20 to 40 weeks of gestation and people from birth to 85
years were examined. The content of positively stained dermal
fibroblasts and the expression level of lamins A, B1 and B2 in their
nucleus was explored by immunohistochemistry. Results and discussion.
The gradual decrease of fibroblasts with positive staining for lamin A
and the expression level of lamin A after antenatal period and up to old
age depends on the age, but does not have differences, which are
related to gender. The lamin B1 positively fibroblasts content is
declining reliably from birth to 40 years and then it is increasing as
well as the expression level of lamina B1. The indicated changes don’t
have reliable variances in gender. The change in the number of lamina
B2-positively colored fibroblasts and the expression level of lamina B2
has no statistically valid correlation with gender and age. Thus, there
are no gender differences in dynamical age related changes of the
content and the expression level of protein-lamina in skin fibroblasts
in a process of chronological aging.
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