ADRENO- AND CHOLINOREACTIVITY OF ERYTHROCYTES AND ESTIMATION OF THEIR CONJUGATION WITH PARAMETERS OF FREE RADICAL BALANCE OF BLOOD IN NON-LINEAR RATS
Evgeniya Vladimirovna KURYANOVA, Andrey Valerievich TRYASUCHEV, David Lvovich TEPLY
The Astrakhan State University
Keywords: адрено- и холинореактивность эритроцитов, свободнорадикальный баланс крови, анаприлин, атропин, нелинейные крысы, adreno- and cholinoreactivity of erythrocytes, free radical balance of blood, anapriline, atropine, non-linear rats
Abstract
The values of the
β-adrenoreactivity of erythrocytes (β-ARE, 40.6 ± 2.3 rel. units) and
the M-cholinoreactivity of erythrocytes (M-ChRE, 7.9 ± 0.3 rel. units),
concentration of hemoglobin, the parameters of free radical balance: the
level of TBA-reactive products (TBA-RP) and catalase activity in
erythrocytes and plasma were determined in male non-linear rats. Sample
grouping by clustering largest β-ARE showed that rats with a high β-ARE
(>50 rel. units) have a greater concentration of hemoglobin, TBA-RP
and CAT in erythrocytes, than rats with an average (30 to 50 rel. units)
and low (<30 rel. units) β-ARE. According to the results of the
correlation and factor analysis, M-ChRE is predominantly conjugated with
erythrocyte (Factor 1), and β-ARE - with plasma characteristics of free
radical balance in blood (Factor 3). The introduction of β-adrenergic
blocker anapriline (2 mg/kg bw) decreases β-ARE and potentiates the
accumulation of TBA-RP in the erythrocytes and plasma, the
administration of the blocker of M-cholinergic receptors atropine (1
mg/kg bw) reduces not only M-ChRE, but also β-ARE and promotes the
growth of erythrocyte catalase activity. Thus, in the intact state,
under the conditions of blockade of adrenergic receptor and cholinergic
receptors, dependences have been revealed that make it possible to treat
β-ARE and M-ChRE as a link between regulatory influences on
erythrocytes and biochemical, including free radical processes in the
«erythrocyte - blood plasma».
|
