Home – Home – Jornals – The journal "Ateroskleroz" 2019 number 1

Objective was to study the levels and the relationship between lipoprotein-associated phospholipase A2 (Lp-PLA2) and proprotein convertase subtilisin/kexin type 9 (PCSK9), their relationship with other lipid parameters in patients with familial hypercholesterolemia (FH) in Russia. Material and methods. The study involved 47 patients (11 males) observed in the Scientific-clinical center of lipidology of Research Institute of Internal and Preventive Medicine - Branch of the Institute of Cytology and Genetics of SB RAS with diagnosis of «possible» or «definite» FH according to the criteria of The Simon Broome Register Group and «probable» or «definite» FH according to the Dutch Lipid Clinic Network Criteria. Patient age was 49.13 ± 12.67 years (mean ± SD); 50.00 [40.00; 59.00] years (median [lower quartile; upper quartile]). Lp-PLA2 and PCSK9 content was determined by enzyme-linked immunosorbent assay using a test systems «Human Proprotein Convertase 9/PCSK9 Immunoassay» («R&D Systems», USA) and «ELISA Kit for Phospholipase A2 Group VII (LpPLA2)» («Cloud-Clone Corp.», USA). Results. Lp-PLA2 level was 73,69 [64,99; 106,53] ng/ml, PCSK9 – 352,16 [272,94; 416,79] ng/ml. There was a trend to moderate positive correlation between Lp-PLA2 and PCSK9 content (r = 0.614; p = 0.059), the statistical significance value was borderline due to the small number surveyed. Males showed a strong positive correlation of LP-FLA2 concentration with age (r = 0.746; p = 0.008), partial – with triglyceride (TG) content (r = 0.793; p = 0.019). A strong positive correlation of PCSK9 level with apolipoprotein B / apolipoprotein A ratio (r = 0.702; p = 0.007) and a weak positive correlation with TG concentration (r = 0.330; p = 0.033) were shown in the general group. Moderate positive correlation of PCSK9 content with age (r = 0.660; p = 0.038), a strong negative correlation with total cholesterol (r = –0.815; p = 0.004), low-density lipoprotein cholesterol (r = –0.828; p = 0.006) and non-high-density lipoprotein cholesterol (r = –0.851, p = 0.002) concentration were shown. Women with TG level <1.7 mmol/l had lower PCSK9 level then women with TG level ≥1.7 mmol/l (328.45 [231.02; 387.82 and 397.12 [348.45; 531.62] ng/ml, respectively (p = 0.013)). Conclusion. The correlation of PCSK9, the perspective marker of cardiovascular diseases, with the avowed marker Lp-PLA2 confirms the importance of PCSK9 in lipid metabolism and cardiovascular homeostasis and is the basis for its further research as a biological marker of cardiovascular diseases.