MULTICHANNEL CONFOCAL MICROSCOPE BASED ON A DIFFRACTION FOCUSING MULTIPLIER WITH AUTOMATIC SYNCHRONIZATION OF SCANNING
V. P. Bessmeltsev, M. V. Maksimov, V. V. Vileiko, N. V. Goloshevskii, V. S. Terent'ev
Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, pr. Akademika Koptyuga 1, Novosibirsk, 630090 Russia
Keywords: многоканальный конфокальный микроскоп, дифракционный оптический элемент, сдвиговые плоскопараллельные пластины, multichannel confocal microscope, diffractive optical element, shifted plane-parallel plates
Abstract
Implementation of a laser scanning confocal microscope is described, where the specimen is scanned by an array of illuminating beams, which significantly increases the velocity of object image construction. The array formation is provided by using a diffractive optical element. Scanning by the array of laser beams over the specimen is performed by galvanometric scanners with moving refractive plane-parallel plates. Owing to application of such a scanning device, the beams in the illuminating channel and the signal beams in the receiving channel pass through one motionless array of confocal diaphragms; as a result, the scanning beams in the specimen plane and the signal beams in the plane of the photodetector matrix can be used without an additional synchronized pair of scanners. The proposed confocal microscope can be applied in problems that require a fast response
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