A PANEL OF GENETIC MARKERS FOR ANALYZING THE RISK OF LONG-TERM ADVERSE PROGNOSIS OF CARDIOVASCULAR DISEASES
E.V. Shakhtshneider1, P.S. Orlov1,2, L.V. Shcherbakova1, D.E. Ivanoshchuk1,2, S.K. Malyutina1, V.N. Maksimov1,2, V.V. Gafarov1, M.I. Voevoda1,2
1Institute of Internal and Preventive Medicine - Branch of Federal Research Institute of Cytology and Genetics of SB RAS, 630089, Novosibirsk, Boris Bogatkov str., 175/1
2Federal Research Center Institute of Cytology and Genetics of SB RAS, 630090, Novosibirsk, Academician Koptyug av., 2
Keywords: генетический рискометр, неблагоприятные исходы, инфаркт миокарда, APOE, CETP, сердечно-сосудистые заболевания, model of genetic risk, unfavorable outcomes, myocardial infarction, APOE, CETP, cardiovascular diseases
Abstract
Aim: to form a panel of genetic markers for analyzing the risk of long-term adverse prognosis of cardiovascular diseases in Russia population. Material and methods. A sample of nine hundred individuals was formed within the framework of the HAPIEE project (age 45-69 years, middle age 53.9 ± 6.9, male/female - 50/50). Molecular genetic analysis was performed using PCR followed by restriction fragment length polymorphism analysis for the APOC3 gene rs5128 polymorphism, APOE gene rs429358 and rs7412, CETP gene rs708272, LPL gene rs320, SCARB1 gene rs5888, and SREBF2 gene rs2228314 . Results. 10 single nucleotide polymorphisms were analyzed for the inclusion in the risk model of adverse prognosis of cardiovascular diseases in Russia population. The APOE gene e4/e4 genotype (rs429358 and rs7412) was associated with the development of adverse cardiovascular outcomes for 7 years of observation. For the CETP gene rs708272, the association with the development of cases of fatal myocardial infarction for 7 years of observation was demonstrated for the genotypes carrying C allele. No statistically significant association with the risk of adverse prognosis of cardiovascular diseases was obtained for the APOC3 gene rs5128, LPL gene rs320, SCARB1 gene rs5888, and SREBF2 gene rs2228314 in this pilot study. Conclusions. Pilot data on the presence of statistically significant association with the risk of long-term adverse prognosis of cardiovascular diseases in Russia population were obtained for the APOE and CETP genes.
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