Genetic Сharacteristics of Kodar Snow Heep Using SNP Markers
D. G. MEDVEDEV1,2, A. V. DOTSEV2, I. M. OKHLOPKOV2,3, T. E. DENISKOVA2, H. REYER4, K. WIMMERS4, G. BREM2,5, V. A. BAGIROV2, N. A. ZINOVIEVA2
1Fund for studying, preservation of the snow leopard and the rare species of mountain fauna, 664011, Irkutsk, Proletarskaya str., 9
2L. K. Ernst Institute of Animal Husbandry, 142132, Dubrovitzy, 60
3Institute of biological problems of cryolitozone, SB RAS, 677890, Yakutsk, Lenin ave., 41
4Institute of Genome Biology, Leibniz Institute for Farm Animal Biology Leibnits, Dummerstorf, Vilgelm-Stakhl-Allee, 2
5Institute of Animal Breeding аnd Genetics, University of Veterinаry Medicine, Viennа, A-1210, Veterenаrplats, 1
Keywords: cнежный баран, кодарский, генетический анализ, SNP, snow sheep, Kodar, genetic analysis, SNP
Abstract
For the first time polymorphisms in Kodar snow sheep, KOD ( Ovis nivicola kodarensis ) nuclear DNA were studied. KOD is a representative of small isolated population of Asian snow sheep ( Ovis nivicola Eschscholtz, 1829) inhabiting the Kodar Ridge (Irkutsk Oblast, Transbaikal Krai). We compared KOD with its geographically closest Yakut subspecies ( Ovis nivicola lydekkeri ). Genome-wide study of single-nucleotide polymorphisms (SNPs) was performed using the Illumina OvineSNP50 BeadChip (Illumina Inc., San Diego, CA, USA). The final set of markers for analysis included 1030 SNPs. We found that Kodar snow sheep had almost 10 times lower level of genetic diversity evaluated by multilocus heterozygosity - MLH (0.027 for KOD - 0.215-0.270 for individuals of Yakut subspecies) and standardized MLH - stMLH (0.116 against 0.910-01.147). The results of multidimensional scaling (MDS), Nei distances calculations ( DN ) and STRUCTURE analysis showed a clear genetic differentiation of Kodar snow sheep from Yakut subspecies. Our data represent the first step to understanding of the demographic history of the original Kodar population of snow sheep.
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