HYPOLIPIDEMIC AND ANTITHROMBOTIC EFFECT HEPARINOID FROM PEONY IN METABOLIC SYNDROME
T.Yu. Obergan, M.G. Lyapina, M.S. Uspenskaya, L.A. Lyapina
Moscow State University n. a. M.V. Lomonosov, 119234, Moscow, Leninskie Gory, 1, bld. 12
Keywords: plant anticoagulant depolymerization of fibrin, lipid profile, fibrinolysis
Abstract
The aim of this work was to study the combined effect on lipid metabolism (total cholesterol, low density lipoprotein, triglycerides) and hemostatic system anticoagulant from plant peony. Material and methods. The vegetable anticoagulantfrom roots of a peony Marin root ( P. anomala ) obtained by the authors was purified from proteins and standardized by koagulological, metachromatical and spectral methods, and determined its activity is 146.1 IU heparin(оid) in 1 ml. Structural features of the drug is established by the method of IR-spectroscopy. The experiments were performed in an experimental model of rats with metabolic syndrome. All animal experiments were conducted in accordance with ethical principles and documents recommended by the European Convention for the protection of vertebrate animals (Strasbourg 15.06.2006). Vegetable anticoagulant in the form of a 5 % extract containing heparin-like component was administered repeatedly (for 7 days) oral administration to animals (rats) at a daily dose 14.61 IU in a volume of 0.1 ml/200 g of body weight. To determine the functional state of hemostasis system was used koagulological standard methods. Determination of lipid profile parameters in blood plasma were measured for total cholesterol, high density lipoproteins (HDLP), low density lipoprotein (LDLP) and triglycerides. Produced weighing rats before and after the experiment. Results. In the development of the metabolic syndrome the use of herbal heparinoidcontributed hypolipidemic (reduction of triglycerides and LDLP by 33 and 16 %, respectively), anticoagulant (increase in activity of 27 %), fibrinolytic (increased activity of 46 %), fibrindepolymerization (increase in activity of 34 %) effects in the blood while the reduced body mass of animals in comparison with control. Conclusion. The obtained data make a detailed study of action mechanism of plant heparinoid from peony, revealing its other healthful properties, in addition to antithrombotic and lipolyticoperation, when used in minimal doses. This drug may be a promising therapeutic agent and can further be used in the treatment of diseases, including cardiovascular, complicated by thrombosis, and related disorders of lipid metabolism.
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